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January 1, 2003Anesthesia & Analgesia

Extending the Skeletal Muscle Viability Period in the Malignant Hyperthermia Test

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Why the study?

Does clamped-warm storage extend the viability period of skeletal muscle for the malignant hyperthermia test to 24 hours in MHS patients?

Population

20 malignant hyperthermia susceptibility (MHS) patients

Comparison

Storage of surplus skeletal muscle samples in… vs Other storage conditions

Design

Preclinical

Follow-up

22-26 hours

Authors

SBSaiid BinaUniformed Services University of the Health SciencesSHStephen HolmanUniformed Services University of the Health SciencesSMSheila M. MuldoonUniformed Services University of the Health Sciences

Discussion

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Implication

May extend MH biopsy viability to 24 h in animal models; leaves open human translation and interlaboratory use.

Structured PICO

Does clamped-warm storage extend the viability period of skeletal muscle for the malignant hyperthermia test to 24 hours in MHS patients?

P
Population
20 malignant hyperthermia susceptibility (MHS) patients
I
Intervention
Storage of surplus skeletal muscle samples in Krebs buffer (pH 7.4) at 23°C–25°C under tension (clamped-warm) for 22-26 hours
C
Comparator
Other storage conditions (clamped-cold at 4°C, free-warm at 23°C–25°C, free-cold at 4°C)
O
Outcome
Response to halothane (3%) and caffeine (0.5–32 mM) and correct diagnosis of MHS at 22–26 h after excisionsurrogate

Storing skeletal muscle samples under tension in Krebs buffer at 23°C–25°C extends their viability for malignant hyperthermia testing up to 24 hours, potentially allowing for interlaboratory sharing of control samples.

Cite This Study

Bina et al. (2003) studied this question.

synapsesocial.com/papers/6a703dbff44fa9f079ddd1e4https://doi.org/10.1213/00000539-200301000-00031
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Standardization of the Caffeine Halothane Muscle Contracture Test1989 · 242 citations
  2. 2Malignant hyperthermia1992 · 361 citations
  3. 3Prolonging Viability of Swine Muscle Biopsy Specimens in Malignant Hyperthermia Testing2001 · 6 citations
  4. 4A Multicenter Study of 4-Chloro-m-cresol for Diagnosing Malignant Hyperthermia Susceptibility2000 · 47 citations
  5. 5In vitro contracture test for diagnosis of malignant hyperthermia following the protocol of the European MH Group: Results of testing patients surviving fulminant MH and unrelated low‐risk subjects1997 · 303 citations