April 1, 1994Cardiovascular Research

Improved quantification with validation of multiple mRNA species by polymerase chain reaction: application to human myocardial creatine kinase M and B

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Population

42 samples from 24 human hearts, including those with end-stage cardiomyopathies of various aetiologies

Comparison

Quantitative polymerase chain reaction method vs Quantitative S1 nuclease protection assay

Design

Preclinical

Authors

TSTalati Shailesh SBrainstorm (Spain)PBPaul A. BrinkStellenbosch University BPBenjamin PerrymanUniversity of South Dakota

Discussion

Member takes

Implication

qPCR enables multiplex CK mRNA quantification in cardiac tissue; leaves open advantages over S1 assay in human studies.

Structured PICO

Population

42 samples from 24 human hearts, including those with end-stage cardiomyopathies of various aetiologies

Intervention

Quantitative polymerase chain reaction (PCR) method

Comparator

Quantitative S1 nuclease protection assay

Outcome

Quantification of human heart creatine kinase M (CKM) and B (CKB) mRNAsurrogate

A validated quantitative PCR method offers advantages over S1 nuclease protection assay for simultaneous quantification of multiple mRNA species in human heart tissue.

Cite This Study

S et al. (1994) studied this question.

synapsesocial.com/papers/6a70402475498292b709a477 https://doi.org/10.1093/cvr/28.4.464

Topics

Cardiomyopathy Hypertension management Women and heart disease

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Improved quantification with validation of multiple mRNA species by polymerase chain reaction: application to human myocardial creatine kinase M and B

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Topics

Improved quantification with validation of multiple mRNA species by polymerase chain reaction: application to human myocardial creatine kinase M and B

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Topics

Also Consider