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June 16, 2014BMC Veterinary ResearchOpen Access

Detection of serum antibodies to hepatitis E virus in domestic pigs in Italy using a recombinant swine HEV capsid protein

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Why the study?

Does an ORF2-based ELISA and Western blot accurately detect anti-HEV antibodies in healthy pigs compared to a commercial ELISA kit?

Population

111 sera collected from healthy pigs in two Italian regions

Comparison

ELISA and Western blotting methods using a… vs Commercial ELISA kit

Design

Preclinical

Authors

EPEleonora PonterioIBIlaria Di BartoloGOGinevra Orrù

Discussion

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Overview

Supports ORF2 ELISA for sensitive HEV serosurveillance in pigs; leaves open specificity for confirmatory use.

Structured PICO

Does an ORF2-based ELISA and Western blot accurately detect anti-HEV antibodies in healthy pigs compared to a commercial ELISA kit?

P
Population
111 sera collected from healthy pigs in two Italian regions
I
Intervention
ELISA and Western blotting methods using a truncated capsid (ORF2) protein lacking the first 111 amino acids of a swine HEV genotype 3 strain
C
Comparator
Commercial ELISA kit
O
Outcome
Detection of anti-HEV IgG antibodies (sensitivity and specificity)surrogate

A newly developed ORF2-based ELISA and Western blot demonstrated high sensitivity for detecting anti-HEV antibodies in swine sera, confirming high HEV prevalence in Italian pigs.

Cite This Study

Ponterio et al. (2014) studied this question.

synapsesocial.com/papers/6a704f55e5469ee92be10dbahttps://doi.org/10.1186/1746-6148-10-133
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Development of a New Multispecies Protein A-Based ELISA for Serodiagnosis of Hepatitis E Infection with Validation in Domestic Swine2024
  2. 2Prevalence of antibodies to the hepatitis E virus in pigs from countries where hepatitis E is common or is rare in the human populationThis article is a US Government work and, as such, is in the public domain in the United States of America1999 · 1 citations
  3. 3Screening for Hepatitis E virus (HEV) in slaughtered pig for meat in Uyo using both serological and molecular methods and assessing their public health appraisal2024
  4. 4Hepatitis E Virus RNA Detection in Liver and Muscle Tissues Sampled from Home Slaughtered Domestic Pigs in Central Italy2024
  5. 5Genetic Heterogeneity of Hepatitis E Virus (HEV) in Wild Boars in Italy2026