Functional reconstitution in lipid vesicles of influenza virus M2 protein expressed by baculovirus: evidence for proton transfer activity

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Population

Spodoptera frugiperda Sf9 cells expressing influenza virus M2 protein, reconstituted into artificial…

Comparison

Imposition of an ionic gradient and treatment… vs Amantadine-resistant mutant M2 (M2 delta)

Design

Preclinical

Authors

Discussion

Supports M2 as rimantadine-sensitive proton channel target; leaves open clinical translation and in vivo validation.

Population

Spodoptera frugiperda Sf9 cells expressing influenza virus M2 protein, reconstituted into artificial membrane vesicles

Intervention

Imposition of an ionic gradient and treatment with rimantadine or N-ethyl-rimantadine

Comparator

Amantadine-resistant mutant M2 (M2 delta)

Outcome

Intravesicular pH decrease (proton translocation)surrogate

Isolated influenza virus M2 protein functions as an ion channel and demonstrates in vitro M2-mediated proton translocation.

Schroeder et al. (1994) studied this question.

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