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December 1, 2010Gut PathogensOpen Access

Luminal and mucosal-associated intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome

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Authors

ICIan M. CarrollUniversity of North Carolina at Chapel Hill YCYoung-Hyo ChangKorea Research Institute of Bioscience and Biotechnology JPJiwon ParkSt. Mary's Hospital

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Implication

Case-control study reveals altered fecal bacterial profiles in diarrhea-predominant irritable bowel syndrome, indicating distinct microbial dysbiosis.

Key Points

To investigate fecal and mucosal-associated intestinal microbiota alterations in patients with diarrhea-predominant irritable bowel syndrome compared to healthy controls using culture and molecular techniques.
Enrolled 10 patients with diarrhea-predominant irritable bowel syndrome (D-IBS) and 10 healthy controls.
Collected fecal samples and colonic mucosal biopsies via un-sedated, un-prepped flexible sigmoidoscopy.
Evaluated bacterial groups via aerobic and anaerobic culture alongside quantitative real-time PCR (qPCR) enumeration on extracted DNA.
Culture analysis demonstrated a significant reduction in fecal aerobic bacteria in D-IBS compared to healthy controls (1.4 × 10^7 vs. 8.4 × 10^8 CFUs/g feces, P = 0.002).
qPCR analysis revealed a significant 3.6-fold increase in fecal Lactobacillus species concentrations in D-IBS patients compared with healthy controls (P = 0.02).

Cite This Study

Carroll et al. (2010) studied this question.

synapsesocial.com/papers/6a7062e3f44fa9f079de03c2 https://doi.org/10.1186/1757-4749-2-19

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Implication

Case-control study reveals altered fecal bacterial profiles in diarrhea-predominant irritable bowel syndrome, indicating distinct microbial dysbiosis.

Luminal and mucosal-associated intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome

Discussion

Member takes

Implication

Key Points

Cite This Study

Luminal and mucosal-associated intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome

Discussion

Member takes

Implication

Key Points

Cite This Study

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