What question did this study set out to answer?

To characterize and compare the subcellular distribution of actin across stages of the cell cycle using affinity-purified anti-actin antibodies and fluorescent heavy meromyosin.

March 1, 1979The Journal of Cell BiologyOpen Access

Comparison of purified anti-actin and fluorescent-heavy meromyosin staining patterns in dividing cells.

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Population

Cultured cells (HeLa cells, PtK-2 cells) and myofibrils

Comparison

Purified anti-actin antibodies vs Fluorescent-heavy meromyosin

Design

Preclinical

Authors

IHIra M. HermanTufts University TPT.D. PollardUniversity of Maryland, Baltimore

Discussion

Member takes

Implication

Should not change clinical practice; leaves open actin organization during mitosis in mammalian cells.

Key Points

To characterize and compare the subcellular distribution of actin across stages of the cell cycle using affinity-purified anti-actin antibodies and fluorescent heavy meromyosin.
Purified anti-actin antibodies via affinity chromatography from serum of rabbits immunized with glutaraldehyde-fixed chicken gizzard actin filaments.
Assessed actin distribution in myofibrils, HeLa cells, and PtK-2 cells across the cell cycle using direct and indirect immunofluorescence.
Compared anti-actin localization patterns directly with fluorescent heavy meromyosin staining in fixed cultured cells.
Anti-actin reacted specifically with smooth and skeletal muscle actin and nonmuscle actin, labeling myofibril I-bands, membrane ruffles, and stress fibers, with direct immunofluorescence yielding zero detectable nonspecific background.
During metaphase and anaphase, anti-actin concentrated diffusely within the mitotic spindle, whereas fluorescent heavy meromyosin resolved into discrete, fine spindle fibers.
Throughout cytokinesis, anti-actin stained the cytoplasm uniformly and showed no preferential concentration at the cleavage furrow.

Structured PICO

Population

Cultured cells (HeLa cells, PtK-2 cells) and myofibrils

Intervention

Purified anti-actin antibodies (using direct or indirect fluorescent antibody technique)

Comparator

Fluorescent-heavy meromyosin

Outcome

Staining patterns in dividing cells at each stage of the cell cyclesurrogate

Anti-actin and fluorescent-heavy meromyosin exhibit distinct staining patterns in the mitotic spindle of dividing cells.

Cite This Study

Herman et al. (1979) studied this question.

synapsesocial.com/papers/6a706500febe604dd7092e35 https://doi.org/10.1083/jcb.80.3.509

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Comparison of purified anti-actin and fluorescent-heavy meromyosin staining patterns in dividing cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Comparison of purified anti-actin and fluorescent-heavy meromyosin staining patterns in dividing cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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