What question did this study set out to answer?

To analyze the characteristics of normal human megakaryocytes using flow cytometry.

May 1, 1988BloodOpen Access

Flow cytometric analysis of normal human megakaryocytes

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Flow cytometry reveals megakaryocyte characteristics, including size and ploidy, from human bone marrow analysis.

To analyze the characteristics of normal human megakaryocytes using flow cytometry.
Enriched megakaryocytes from bone marrow aspirates using Percoll density gradient.
Labeled cells with fluoresceinated antibodies targeting GPIIb/IIIa for analysis.
Employed flow cytometry to evaluate size and ploidy through forward and side light scatter.
Mean diameter of megakaryocytes was 28.1 +/- 12.3 microns.
Ploidy distribution was predominantly 16N, with one-fifth of cells ≤ 4N.
Total membrane GPIIb/IIIa expression increased with cell size and ploidy (r = 0.99).

Tomer et al. (1988) studied this question.

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