The Gene and Protein Expression of the Main Components of the Lipolytic System in Human Myocardium and Heart Perivascular Adipose Tissue. Effect of Coronary Atherosclerosis

Figures

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  Figure 1. Myocardial and perivascular adipose tissue proteins expression of (A) adipose triglyceride lipase (ATGL), (B) comparative gene identification 58 (CGI-58), (C) G0/G1 switch gene 2 (G0S2), (D) hormone-sensitive lipase (HSL) in the coronary atherosclerosis (CAD, n = 42) and control (NCAD, n = 11) patients. Representative bands of WB analysis were shown. The inner horizontal line of a box represents the median. Box boundaries: 25–75 percentile; whiskers 5–95 percentile. Data are expressed as median ± SEM. For the sake of clarity, the control group median was set at 100, and the CAD group was scaled with respect to NCAD. * p < 0.05 vs. control subjects.

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  Figure 2. Myocardial and perivascular adipose tissue genes expression of (A) adipose triglyceride lipase (ATGL), (B) comparative gene identification 58 (CGI-58), (C) G0/G1 switch gene 2 (G0S2), (D) hormone-sensitive lipase (HSL) in the coronary atherosclerosis (CAD, n = 42) and control (NCAD, n = 11) patients. The inner horizontal line of a box represents the median. Box boundaries: 25–75 percentile; whiskers 5–95 percentile. Data are expressed as median ± SEM. For the sake of clarity, the control group median was set at 100, and the CAD group was scaled with respect to NCAD. * p <0.05 vs. control subjects.

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  Figure 3. Myocardial and perivascular adipose tissue genes expression of (A) beta-hydroxyacyl CoA dehydrogenase (β-HAD), (B) citrate synthase (CS), (C) cytochrome c oxidase subunit IV isoform 1 (COX4/1) in the coronary atherosclerosis (CAD, n = 42) and control (NCAD, n = 11) patients. The inner horizontal line of a box represents the median. Box boundaries: 25–75 percentile; whiskers 5–95 percentile. Data are expressed as median ± SEM. For the sake of clarity, the control group median was set at 100, and the CAD group was scaled with respect to NCAD. * p < 0.05 vs. control subjects.

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  Figure 4. Myocardial and perivascular adipose tissue proteins expression of (A) beta-hydroxyacyl CoA dehydrogenase (β-HAD), (B) citrate synthase (CS), (C) cytochrome c oxidase subunit IV isoform 1 (COX4/1) in the coronary atherosclerosis (CAD, n = 42) and control (NCAD, n = 11) patients. Representative bands of WB analysis were shown. The inner horizontal line of a box represents the median. Box boundaries: 25–75 percentile; whiskers 5–95 percentile. Data are expressed as median ± SEM. For the sake of clarity, the control group median was set at 100, and the CAD group was scaled with respect to NCAD. * p < 0.05 vs. control subjects.

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  Figure 5. Myocardial and perivascular adipose tissue genes expression of (A) fatty acid translocase (FAT/CD36), (B) fatty acid-binding protein 4 (FABP-4), (C) glycerol-3-phosphate acyltransferase 1 (GPAT1), (D) lipoprotein lipase (LPL) in the coronary atherosclerosis (CAD, n = 42) and control (NCAD, n = 11) patients. The inner horizontal line of a box represents the median. Box boundaries: 25–75 percentile; whiskers 5–95 percentile. Data are expressed as median ± SEM. For the sake of clarity, the control group median was set at 100, and the CAD group was scaled with respect to NCAD. * p < 0.05 vs. control subjects.

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  Figure 6. Myocardial and perivascular adipose tissue proteins expression of (A) fatty acid translocase (FAT/CD36), (B) fatty acid-binding protein 4 (FABP-4) in the coronary atherosclerosis (CAD, n = 42) and control (NCAD, n = 11) patients. Representative bands of WB analysis were shown. The inner horizontal line of a box represents the median. Box boundaries: 25–75 percentile; whiskers 5–95 percentile. Data are expressed as median ± SEM. For the sake of clarity, the control group median was set at 100, and the CAD group was scaled with respect to NCAD. * p < 0.05 vs. control subjects.

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  Figure 7. Myocardial proteins expression of (A) fatty acid synthase (FAS), (B) sterol-regulatory-element-binding protein-1 (SREBP-1c) and perivascular of (C) glycerol-3-phosphate acyltransferase 1 (GPAT1) in the coronary atherosclerosis (CAD, n = 42) and control (NCAD, n = 11) patients. Representative bands of WB analysis were shown. The inner horizontal line of a box represents the median. Box boundaries: 25–75 percentile; whiskers 5–95 percentile. Data are expressed as median ± SEM. For the sake of clarity, the control group median was set at 100, and the CAD group was scaled with respect to NCAD. * p < 0.05 vs. control subjects.

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  Figure 8. Myocardial proteins expression of (A) Cyclooxygenase-2 (COX-2), (B) lipoxygenase (15-LO), (C) nuclear factor kappa B (NFκβ) in the coronary atherosclerosis (CAD, n = 24) and control (NCAD, n = 11) patients. The number of subjects with atherosclerosis studied was smaller than the number assayed previous determinations due to an insufficient amount of the muscle tissue available. Representative bands of WB analysis were shown. The inner horizontal line of a box represents the median. Box boundaries: 25–75 percentile; whiskers 5–95 percentile. Data are expressed as median ± SEM. For the sake of clarity, the control group median was set at 100, and the CAD group was scaled with respect to NCAD. * p < 0.05 vs. control subjects.

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  Figure 9. Myocardial content of (A) free fatty acids (FFA), (B) diacylglycerols (DG) and (C) triacylglycerols (TG) in the coronary atherosclerosis (CAD, n = 24) and control (NCAD, n = 11) patients. The number of subjects with atherosclerosis studied was smaller than the number assayed for mRNA and protein expression due to the insufficient amount of the muscle tissue available. Data are expressed as mean ± SEM. * p < 0.05 vs. control subjects.

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  Figure 10. Correlations of ATGL expression and TG content in human myocardium of (A) NCAD and (B) CAD patients.