Key result
Under long-term high-fat diet conditions, ACE2 deficiency in mice significantly increased glucose area under the curve and decreased islet microvessel density and insulin content (all P < 0.05).
Why the study?
Does ACE2 deficiency aggravate glucose intolerance and impair islet microvascular density in mice on a high-fat diet?
Does ACE2 deficiency aggravate glucose intolerance and impair islet microvascular density in mice on a high-fat diet?
p-value: p=< 0.05
ACE2 deficiency deteriorates islet function and aggravates glucose intolerance in mice on a long-term high-fat diet by impairing islet microvasculature.
ACE2 deficiency impairs islet microvasculature and glucose tolerance in high-fat-fed mice; leaves open translation to human diabetes therapies.
The aim of this study was to evaluate the effects of angiotensin-converting enzyme 2 (ACE2) on glucose homeostasis and islet function in mice. Male wildtype (WT) and ACE2 knockout (ACE2 KO) mice were divided into chow diet group and long-term high-fat diet (HFD) group. After 16 weeks of feeding, the islet function of the animals was evaluated by intraperitoneal glucose tolerance test (IPGTT) and intraperitoneal insulin releasing test (IPIRT). The pancreas was immunohistochemically stained to analyze the relative content of insulin (IRC), vascular endothelial growth factor (VEGF), and microvessel density (MVD) in islets. There was no difference of body weight, area under curve of glucose (AUCG), area under curve of insulin from 0 to 5 min (AUGI0-5), MVD, and RVC (relative content of VEGF) between WT and ACE2 KO mice with regular chow diet. Under the condition of long-term HFD, the AUCG of ACE2 KO mice was increased obviously in comparison with the WT mice, with decreased IRC, MVD, AUGI0-5, AUCI0-30, and RVC (all P < 0.05). In conclusion, these results show that ACE2 deficiency deteriorates islet function of mice with long-term HFD via impairment of islet microvasculature.
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Li et al. (2013) studied Glucose intolerance. ACE2 deficiency (ACE2 KO) vs. Wildtype (WT) mice was evaluated on Glucose homeostasis and islet function (AUCG, IRC, MVD, AUGI0-5, AUCI0-30, RVC) (p=< 0.05). Under long-term high-fat diet conditions, ACE2 deficiency in mice significantly increased glucose area under the curve and decreased islet microvessel density and insulin content (all P < 0.05).
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