Key result
The transition from post-natal to adulthood in mice is associated with significant transcriptional changes, including 1215 upregulated and 2549 downregulated genes, alongside a progressive decline in ejection fraction.
Why the study?
The study aimed to investigate whether the myocardium experiences transcriptional changes during the transition from postnatal to adulthood stages.
p-value: p=<0.05
Mouse data link postnatal transcriptional shifts to EF decline; leaves open relevance to human cardiac aging.
The development of the heart follows a synergic action of several signaling pathways during gestational, pre- & postnatal stages. The current study aimed to investigate whether the myocardium experiences transcriptional changes during the transition from post-natal to adult hood stages. Herein, we used C57/Bl6/J mice at 4 (28-days; post-natal/PN) and 20 weeks (adulthood/AH) of ages and employed the next generation RNAseq (NGS) to profile the transcriptome and echocardiography analysis to monitor the structural/functional changes in the heart. NGS-based RNA-seq revealed that 1215 genes were significantly upregulated and 2549 were down regulated in the AH versus PN hearts, indicating a significant transcriptional change during this transition. A synchronized cardiac transcriptional regulation through cell cycle, growth hormones, redox homeostasis and metabolic pathways was noticed in both PN and AH hearts. Echocardiography reveals significant structural and functional (i.e. systolic/diastolic) changes during the transition of PN to adult stage. Particularly, a progressive decline in ejection fraction (EF) and cardiac output was observed in AH hearts. These structural adaptations are in line with critical signaling pathways that drive the maturation of heart during AH. Overall, we have presented a comprehensive transcriptomic analysis along with structural-functional relationship during the myocardial development in adult mice.
No takes yet. Share an insight, caveat, or question.
Sunny et al. (2020) studied Normal cardiac development (n=30). Adulthood (20 weeks of age) vs. Post-natal (4 weeks of age) was evaluated on Differential gene expression in the myocardium (p=<0.05). The transition from post-natal to adulthood in mice is associated with significant transcriptional changes, including 1215 upregulated and 2549 downregulated genes, alongside a progressive decline in ejection fraction.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: