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Real-time PCR provides a rapid, reliable, and cost-effective alternative to MS-PCR for detecting angiotensinogen gene polymorphisms associated with cardiovascular disease.
OBJECTIVE: to develop a rapid and reliable real-time PCR to detect polymorphisms of angiotensinogen (AGT), to compare the two methods of MS-PCR (Mutagenically Separated PCR) and real-time PCR to determine three polymorphisms of the angiotensinogen gene M235T, the A (-6) G and A (-20) C. METHODS: the method of real-time PCR was developed on the PLC Roche LightCycler1 with SYBR Green I. We used two sense primers and a primer nonsense. Detection of polymorphisms of angiotensinogen gene was performed by comparing the melting curves. methods: real-time PCR and MS-PCR. In our study, no differences were found between the two techniques. DISCUSSION: The real-time PCR is a rapid and reliable method for detecting gene polymorphisms on the AGT M235T, the A (-6) G and A (-20) C. CONCLUSION: this method of real-time PCR is a reliable genetic test, which is fast and cheap and can be used in practice to study particular polymorphisms of AGT gene associated with cardiovascular disease.
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Dandana et al. (2011) studied this question.
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