Key result
ACE I/D polymorphisms show no association with coronary atherosclerotic heart disease or ischemic stroke.
Why the study?
Angiotensin-converting enzyme plays a major role in blood pressure regulation and cardiovascular homeostasis, with its wide distribution and multifunctional properties suggesting involvement in various pathophysiological conditions.
Can a novel direct PCR-LFA biosensor accurately and rapidly detect ACE I/D polymorphisms in clinical samples?
Case-Control (n=633)
No
Can a novel direct PCR-LFA biosensor accurately and rapidly detect ACE I/D polymorphisms in clinical samples?
p-value: p=0.53
A novel direct PCR-LFA biosensor provides rapid and accurate detection of ACE I/D polymorphisms, which may aid in diagnosing diseases associated with large fragment gene insertions/deletions.
No association of ACE I/D polymorphisms with CAD or stroke; leaves open their role in risk stratification and requires larger validation studies.
BACKGROUND: Angiotensin-converting enzyme (ACE) plays a major role in blood pressure regulation and cardiovascular homeostasis. The wide distribution and multifunctional properties of ACE suggest it's involvement in various pathophysiological conditions. RESULTS: In this study, a novel visual detection method for ACE I/D polymorphisms was designed by integrating direct PCR without the need for DNA extraction using gold magnetic nanoparticles (GMNPs)-based lateral flow assay (LFA) biosensor. The entire detection procedure could enable the genotyping of clinical samples in about 80 min. The detection limit was 0.75 ng and results could be obtained in 5 min using the LFA device. Three hundred peripheral blood samples were analyzed using the direct PCR-LFA system and then verified by sequencing to determine accuracy and repeatability. A clinical preliminary study was then performed to analyze a total of 633 clinical samples. CONCLUSIONS: After grouping based on age, we found a significant difference between the genotypes and the age of patients in the CHD group. The introduction of this method into clinical practice may be helpful for the diagnosis of diseases caused by large fragment gene insertions/deletions.
No takes yet. Share an insight, caveat, or question.
Yang et al. (2021) conducted a case-control in Coronary atherosclerotic heart disease and cerebral ischemic stroke (n=633). ACE I/D polymorphism vs. Control group was evaluated on Association of ACE I/D genotype with coronary atherosclerotic heart disease (p=0.53). ACE I/D polymorphisms were not significantly associated with coronary atherosclerotic heart disease (p=0.53) or cerebral ischemic stroke (p=0.84) compared to healthy controls.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: