Avidin was crystallized in various forms from 3m-ammonium sulphate or sodium phosphate at pH5.2. X-ray-crystallographic analysis of crystals from phosphate showed them to be orthorhombic, space group C222, containing two molecules per unit cell and with a single subunit as the asymmetric unit. The formation of the complex with biotin did not affect the morphology of the crystals.
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Green et al. (1970) studied this question.
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