December 17, 2011AJP Heart and Circulatory Physiology
Overexpression of angiopoietin-2 impairs myocardial angiogenesis and exacerbates cardiac fibrosis in the diabetic db/db mouse model
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Why the study?
Does overexpression of Ang-2 impair myocardial angiogenesis and exacerbate cardiac fibrosis in diabetic db/db mice?
Population
Diabetic db/db male mice, C57BL/6J mice, db/+ mice, and cultured mouse heart microvascular endothelial cells.
Comparison
Intramyocardial administration of adenovirus… vs Intramyocardial administration of Ad-β-gal; in…
Design
Preclinical
Follow-up
48 hours or 14 days after intramyocardial viral transfection
Authors
Discussion
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Implication
Ang-2 may promote microvascular rarefaction in diabetic hearts; hypothesis-generating for targeted inhibition pending human validation.
Structured PICO
Does overexpression of Ang-2 impair myocardial angiogenesis and exacerbate cardiac fibrosis in diabetic db/db mice?
P
Population
Diabetic db/db male mice (12 wk of age), C57BL/6J (wild type) mice, db/+ mice, and cultured mouse heart microvascular endothelial cells (MHMEC).
I
Intervention
Intramyocardial administration of adenovirus Ang-2 (Ad-CMV-Ang-2) or adenovirus Ang-1 (Ad-Ang-1) (1 x 10^9 platelet-forming units); in vitro exposure to Ang-2 (250 ng/ml) or Ang-2 siRNA.
C
Comparator
Intramyocardial administration of Ad-β-gal; in vitro normal glucose (5 mmol/l) or high glucose (30 mmol/l) without Ang-2.
O
Outcome
Myocardial capillary density, endothelial apoptosis (TUNEL staining), and cardiac fibrosis (Masson's trichrome).surrogate
Overexpression of Ang-2 in diabetic mice impairs myocardial angiogenesis, increases endothelial apoptosis, and promotes cardiac fibrosis, suggesting Ang-2 contributes to microvascular rarefaction in diabetes.
Cite This Study
Chen et al. (2011) studied this question.
Topics
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