Leukemia Inhibitory Factor Activates Cardiac L-Type Ca 2+ Channels via Phosphorylation of Serine 1829 in the Rabbit Ca v 1.2 Subunit
Why the study?
Does leukemia inhibitory factor increase cardiac L-type Ca2+ channel current via phosphorylation of the Ca(v)1.2 subunit in cardiomyocytes?
Population
Cultured adult and neonatal rat cardiomyocytes, and HEK293 cells transfected with wild-type or mutant rabbit…
Comparison
Leukemia inhibitory factor (LIF) stimulation vs Unstimulated cells, cells treated with MEK…
Design
Preclinical
Authors
Discussion
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Implication
May link LIF to calcium dysregulation in heart disease; leaves open human relevance and therapeutic targeting.
Structured PICO
Does leukemia inhibitory factor increase cardiac L-type Ca2+ channel current via phosphorylation of the Ca(v)1.2 subunit in cardiomyocytes?
P
Population
Cultured adult and neonatal rat cardiomyocytes, and HEK293 cells transfected with wild-type or mutant rabbit Ca(v)1.2 subunits
I
Intervention
Leukemia inhibitory factor (LIF) stimulation
C
Comparator
Unstimulated cells, cells treated with MEK inhibitor PD98059, or cells transfected with mutant Ca(v)1.2 (S1829A)
O
Outcome
Phosphorylation of the Ca(v)1.2 subunit and increase in L-type Ca2+ channel current (I(CaL))surrogate
Leukemia inhibitory factor increases cardiac L-type calcium channel current by phosphorylating serine 1829 on the Ca(v)1.2 subunit via the ERK pathway.
Cite This Study
Takahashi et al. (2004) studied this question.