October 1, 1997The Journal of General PhysiologyOpen Access

Sodium Channel Activation Gating Is Affected by Substitutions of Voltage Sensor Positive Charges in All Four Domains

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Population

Rat brain IIA sodium channel alpha subunit cDNA with fast inactivation removed (IFMQ3 mutation)

Design

Preclinical

Authors

KKKris J. KontisUniversity of California, Irvine ARAmir RounaghiUniversity of California, Irvine AGAlan L. GoldinElectrophysiology

Discussion

Member takes

Implication

Domain-specific S4 neutralization effects on NaV activation extend structure-function models; hypothesis-generating for arrhythmia research with no clinical translation yet.

Structured PICO

Population

Rat brain IIA sodium channel alpha subunit cDNA with fast inactivation removed (IFMQ3 mutation)

Intervention

Mutations (charge-neutralizing and -conserving) of the second and fourth conserved positive charges in the S4 segments of all four homologous domains (16 single and 2 double mutants)

Outcome

Voltage dependence and kinetics of activation and deactivationsurrogate

The study demonstrates that both charge and structure in all four S4 segments determine the voltage-dependent activation of the sodium channel.

Cite This Study

Kontis et al. (1997) studied this question.

synapsesocial.com/papers/6a709cc7c2d7c3090826f085 https://doi.org/10.1085/jgp.110.4.391

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Implication

Domain-specific S4 neutralization effects on NaV activation extend structure-function models; hypothesis-generating for arrhythmia research with no clinical translation yet.

Sodium Channel Activation Gating Is Affected by Substitutions of Voltage Sensor Positive Charges in All Four Domains

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Sodium Channel Activation Gating Is Affected by Substitutions of Voltage Sensor Positive Charges in All Four Domains

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider