What question did this study set out to answer?

Investigate how anionic and cationic potentiators of skeletal muscle twitches influence cation binding and ATPase activity in the sarcoplasmic reticulum.

March 1, 1968The Journal of General PhysiologyOpen Access

Effects of Potentiators of Muscular Contraction on Binding of Cations by Sarcoplasmic Reticulum

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Investigate how anionic and cationic potentiators of skeletal muscle twitches influence cation binding and ATPase activity in the sarcoplasmic reticulum.
Isolated sarcoplasmic reticulum membrane fragments from rabbit skeletal muscle.
Assayed active and passive binding of calcium (Ca) and magnesium (Mg) in the presence and absence of ATP under exposure to anions (NO3-, Br-, I-, SCN-) and cations (Zn++, Cd++).
Evaluated the effect of these ionic potentiators on membrane Ca-stimulated ATPase activity.
Both anionic (NO3-, Br-, I-, SCN-) and cationic (Zn++, Cd++) potentiators depressed active Ca binding by the sarcoplasmic reticulum.
Zinc and cadmium exchanged directly for Ca and Mg while inhibiting Ca-stimulated ATPase activity, whereas anions induced Mg replacement of active Ca and increased passive cation binding capacity without inhibiting ATPase activity.

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Implication

In vitro study reveals that twitch potentiators alter cation binding in skeletal sarcoplasmic reticulum, suggesting a mechanism for modified excitation-contraction coupling.

Cite This Study

Arsélio P. Carvalho (1968) studied this question.

synapsesocial.com/papers/6a709d33ce524a4339c454fc https://doi.org/10.1085/jgp.51.3.427

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Implication

In vitro study reveals that twitch potentiators alter cation binding in skeletal sarcoplasmic reticulum, suggesting a mechanism for modified excitation-contraction coupling.

Effects of Potentiators of Muscular Contraction on Binding of Cations by Sarcoplasmic Reticulum

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Discussion

Implication

Cite This Study

Effects of Potentiators of Muscular Contraction on Binding of Cations by Sarcoplasmic Reticulum

Key Points

Discussion

Implication

Cite This Study

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