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April 14, 2020Open Access

Single-cell transcriptomic profiling maps monocyte/macrophage transitions after myocardial infarction in mice

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Why the study?

Monocytes and macrophages play a critical, dual role in post-ischemic cardiac repair, but how they acquire heterogeneous functional phenotypes in the ischemic myocardium remained unclear.

Population

Mice undergoing experimental MI and homeostatic conditions

Comparison

Post-MI states vs homeostatic conditions

Design

Time-series single-cell transcriptome and cell surface epitope analysis

Follow-up

Up to 11 days after MI

Authors

GRGiuseppe RizzoInsermEVEhsan VafadarnejadHelmholtz Institute for RNA-based Infection ResearchPAPanagiota ArampatziUniversity of Würzburg

Discussion

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Implication

Highlights monocyte/macrophage heterogeneity post-MI in mice; leaves open targeted therapies pending human validation.

Structured PICO

P
Population
Mice subjected to experimental myocardial infarction (MI) and sham-operated controls.
I
Intervention
Experimental myocardial infarction (MI)
C
Comparator
Homeostatic conditions (no MI) and sham surgery
O
Outcome
Gene expression dynamics and transitions in circulating and cardiac monocyte/macrophage states at the single-cell levelsurrogate

Single-cell transcriptomic profiling maps the rapid transition of circulating monocytes into distinct, time-dependent macrophage populations (MHCIIhi and Trem2hiIgf1hi) within the ischemic myocardium after myocardial infarction.

Cite This Study

Rizzo et al. (2020) studied this question.

synapsesocial.com/papers/6a709e91ce524a4339c45786https://doi.org/10.1101/2020.04.14.040451
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Differential Contribution of Monocytes to Heart Macrophages in Steady-State and After Myocardial Infarction2014 · 617 citations
  2. 2Sham Surgery and Inter-Individual Heterogeneity Are Major Determinants of Monocyte Subset Kinetics in a Mouse Model of Myocardial Infarction2014 · 12 citations
  3. 3The pericardium promotes cardiac repair and remodelling post-myocardial infarction2019 · 6 citations
  4. 4Time-resolved single-cell transcriptomics uncovers dynamics of cardiac neutrophil diversity in murine myocardial infarction2019 · 12 citations
  5. 5The Gene Ontology Resource: 20 years and still GOing strong2018 · 5,274 citations