Identification and characterization of a HeLa nuclear protein that specifically binds to the trans-activation-response (TAR) element of human immunodeficiency virus.

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Population

HeLa cell extracts and in vitro transcribed human immunodeficiency virus type 1 trans-activation-response…

Comparison

UV cross-linking and competition experiments… vs TAR element RNA mutants

Design

Preclinical

Authors

Discussion

May guide TAR-targeted HIV strategies; leaves open the protein's identity, validation, and therapeutic relevance.

Population

HeLa cell extracts and in vitro transcribed human immunodeficiency virus type 1 trans-activation-response (TAR) element RNA

Intervention

UV cross-linking and competition experiments with partially purified 68-kDa nuclear protein

Comparator

TAR element RNA mutants

Outcome

Binding affinity of the 68-kDa nuclear protein to TAR element RNA and its mutantssurrogate

Identifies a 68-kDa cellular protein that specifically binds the HIV-1 TAR element, suggesting it moderates TAR activity in vivo.

Marciniak et al. (1990) studied this question.

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