Energetic Requirements for Processive Elongation of Actin Filaments by FH1FH2-formins
Population
Single molecules of the formin Bni1p from Saccharomyces cerevisiae labeled with quantum dots
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
Challenges prior profilin-dependent models of formin elongation; leaves open validation in cellular or in vivo contexts.
Structured PICO
P
Population
Single molecules of the formin Bni1p from Saccharomyces cerevisiae labeled with quantum dots
I
Intervention
Profilin and ATP-actin subunits
O
Outcome
Energetic requirements and rates of processive elongation of actin filaments
Actin subunit addition alone likely provides the energy for fast processive elongation of filaments mediated by FH1FH2-formin and profilin, challenging previous proposals that required profilin or ATP hydrolysis.
Cite This Study
Paul et al. (2009) studied this question.