Objective: Adipose-Derived Stem Cells (ADSCs) are less immunogenic cells and have a heterogenic cytokine secretion profile making them a better candidate for cell-based immunotherapy. Even innately or stimulated, Interleukin-6 (IL-6) and Toll-like Receptor 2 (TLR2) secreted by ADSC were modulated and led to different inflammatory mechanism pathways through different inflammatory factors secretion. These properties can be very useful in the treatment of inflammation-associated chronic diseases. To be used, ex-vivo ADSC expansion is a critical issue prior to transplantation or cryopreservation. Functional cell changes have been reported during culture expansion being susceptible to interact with freezing/thawing effects leading to doubt on cell therapeutic outcomes. The aim of this study is to identify the effect of freezing/thawing at the different time point of expansion culture on IL-6 and TLR2 secretion.
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Othmani et al. (2019) studied this question.
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