A+U content rather than a particular nucleotide order determines the specificity of RNase E cleavage.
Population
Escherichia coli mRNA decay model (pACYC184 RNA I)
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Refines bacterial mRNA decay models; leaves open whether A+U rules apply in eukaryotic or cardiovascular transcript regulation.
Structured PICO
P
Population
Escherichia coli mRNA decay model (pACYC184 RNA I)
I
Intervention
Introduction of random mutations into the decanucleotide region at the 5' end of pACYC184 RNA I
O
Outcome
Position of rne-dependent cleavage in vivo and RNase E-mediated cutting in vitro
RNase E cleavage specificity in E. coli is determined by the A+U content and continuity rather than a strict consensus sequence or distance from secondary structures.
Cite This Study
McDowall et al. (1994) studied this question.