January 28, 2008Journal of Biological ChemistryOpen Access

Control of the Ability of Profilin to Bind and Facilitate Nucleotide Exchange from G-actin

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Population

Yeast and human profilin 1 with yeast and mammalian skeletal muscle actins, including hybrid yeast actins…

Design

Preclinical

Authors

KWKuo‐Kuang WenPenn State Milton S. Hershey Medical Center MMMelissa McKaneUniversity of Ulster JHJon C. D. HoutmanUniversity of Iowa

Discussion

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Implication

Actin-profilin energetics differ by species; leaves open relevance to human cardiac cytoskeletal dynamics.

Structured PICO

Population

Yeast (YPF) and human profilin 1 (HPF1) with yeast and mammalian skeletal muscle actins, including hybrid yeast actins containing subdomain 1 (sub1) or subdomain 1 and 2 (sub12) muscle actin residues.

Intervention

Binding and nucleotide exchange facilitation

Outcome

Binding affinity (Kd) and etheno-ATP exchange ratesurrogate

Profilin controls the energetics of its interaction with nonhybrid actin, and interactions between actin subdomains 1 and 2 affect the topography of the profilin binding site.

Cite This Study

Wen et al. (2008) studied this question.

synapsesocial.com/papers/6a70c6d65671bbf00bc2c4dd https://doi.org/10.1074/jbc.m709806200

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Control of the Ability of Profilin to Bind and Facilitate Nucleotide Exchange from G-actin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Control of the Ability of Profilin to Bind and Facilitate Nucleotide Exchange from G-actin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider