Diacylglycerol‐acyltransferase (EC 2.3.1.20) was partially purified and characterized from spinach leaves. The enzyme had a pH optimum of 8.0 and activity was stimulated 2‐fold by the addition of 20 mM Mn 2+ or Mg 2+ . Diolein and dipalmitin were examined as sn ‐1,2‐diacylglycerol substrates. Only diolein gave detectable triacylglycerol synthesis. In addition, the saturation kinetics of the enzyme with 16∶0‐CoA, 18∶0‐CoA and 18∶1‐CoA were examined. The highest apparent, Km was observed with 18∶1‐CoA, the lowest with 16∶0‐CoA. Endogenous spinach leaf glycerolipids were extracted and analyzed. The leaves contained 70 nmol triacylglycerol (g fresh weight) − .
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Martin et al. (1983) studied this question.
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