What question did this study set out to answer?

To identify the specific factors mediating the synergistic proliferation of human vascular smooth muscle cells stimulated by adipokines and oleic acid across different fat depots.

January 1, 2013Mediators of InflammationOpen Access

VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose Tissue

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Population

Human vascular smooth muscle cells and human adipocytes from obese/lean and diabetic/nondiabetic subjects

Comparison

Adipocyte-conditioned medium and oleic acid… vs Control conditions

Design

Preclinical

Authors

RSRaphaela SchlichZero to Three MWMiriam WillemsDüsseldorf University Hospital SGSabrina GreulichDeutsches Diabetes-Zentrum e.V.

Discussion

Member takes

Implication

May implicate VEGF in obesity- and diabetes-related vascular remodeling; hypothesis-generating from animal data and requires clinical validation.

Key Points

To identify the specific factors mediating the synergistic proliferation of human vascular smooth muscle cells stimulated by adipokines and oleic acid across different fat depots.
Treated cultured human vascular smooth muscle cells with adipocyte-conditioned medium from subcutaneous, visceral, and perivascular adipose depots with or without oleic acid.
Evaluated smooth muscle cell proliferation, receptor expression (VEGF-R1 and VEGF-R2), and VEGF secretion in the presence and absence of neutralizing VEGF antibodies.
Adipocyte-conditioned medium stimulated smooth muscle cell proliferation and upregulated VEGF-R1 and VEGF-R2 in proportion to its VEGF content, whereas VEGF neutralization markedly blunted cell proliferation.
Visceral adipose tissue from obese individuals and perivascular adipose tissue from patients with type 2 diabetes released significantly higher levels of VEGF and induced greater smooth muscle cell proliferation than tissue from lean or nondiabetic controls.

Structured PICO

Population

Human vascular smooth muscle cells (VSMC) and human adipocytes (visceral, subcutaneous, perivascular) from obese/lean and diabetic/nondiabetic subjects

Intervention

Adipocyte-conditioned medium (CM) and oleic acid (OA), with or without VEGF neutralization

Comparator

Control conditions (e.g., subcutaneous adipose tissue vs visceral, lean vs obese, nondiabetic vs diabetic)

Outcome

Proliferation of VSMC and VEGF release/expressionsurrogate

VEGF mediates adipocyte-induced proliferation of vascular smooth muscle cells, suggesting a mechanistic link between depot-specific adipose tissue inflammation in obesity/diabetes and vascular dysfunction.

Cite This Study

Schlich et al. (2013) studied this question.

synapsesocial.com/papers/6a70ee79fe4101aa97e05fd5 https://doi.org/10.1155/2013/982458

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Implication

May implicate VEGF in obesity- and diabetes-related vascular remodeling; hypothesis-generating from animal data and requires clinical validation.

VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose Tissue

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose Tissue

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider