What question did this study set out to answer?

This research investigates how metabolites affect rigor tension development in myocardial ischemia.

May 1, 1994Circulation Research

Myocardial ischemic contracture. Metabolites affect rigor tension development and stiffness.

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Population

rat ventricular Triton X-100-skinned fibers

Comparison

stepwise decreasing [Mg-ATP] in the presence or… vs control conditions (pH 7.1, no added P or Mg-ADP)

Design

Preclinical

Authors

RVRenée Ventura‐ClapierHeart Failure & Transplant VVVladimir VekslerInserm

Discussion

Member takes

Implication

May implicate PCr depletion and ADP accumulation in ischemic contracture; leaves open translation to human myocardial stiffness or CK-targeted therapies.

Key Points

This research investigates how metabolites affect rigor tension development in myocardial ischemia.
Assessed rigor tension in rat ventricular Triton X-100-skinned fibers by manipulating Mg(2+)-ATP and PCr levels.
Set diastolic ionic environment with specific concentrations of Ca2+, Mg2+, and ionic strength for experimentation.
Measured changes in pMg(2+)-ATP50 and tension-to-stiffness ratios under various conditions.
Rigor tension significantly affected by PCr withdrawal, with pMg(2+)-ATP50 shifting to higher values without PCr (3.57 +/- 0.03).
Acidic pH elevated pMg(2+)-ATP50 in both conditions, indicating increased rigor tension response at lower pH levels (P < .001).
Mg(2+)-ADP presence decreased pMg(2+)-ATP50 (3.26 +/- 0.06), indicating its potent influence on tension generation.

Structured PICO

Population

rat ventricular Triton X-100-skinned fibers

Intervention

stepwise decreasing [Mg(2+)-ATP] in the presence or absence of 12 mmol/L phosphocreatine (PCr), with varying levels of inorganic phosphate (P(i)), protons (acidic pH 6.6), and Mg(2+)-ADP

Comparator

control conditions (pH 7.1, no added P(i) or Mg(2+)-ADP)

Outcome

pMg(2+)-ATP for half-maximal rigor tension (pMg(2+)-ATP50) and maximal rigor tensionsurrogate

Ischemic contracture and increased myocardial stiffness may be mediated by decreased phosphocreatine and local Mg(2+)-ADP accumulation, emphasizing the importance of myofibrillar creatine kinase activity.

Cite This Study

Ventura‐Clapier et al. (1994) studied this question.

synapsesocial.com/papers/6a70f96cf44fa9f079debaf8 https://doi.org/10.1161/01.res.74.5.920

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Implication

May implicate PCr depletion and ADP accumulation in ischemic contracture; leaves open translation to human myocardial stiffness or CK-targeted therapies.

Myocardial ischemic contracture. Metabolites affect rigor tension development and stiffness.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Myocardial ischemic contracture. Metabolites affect rigor tension development and stiffness.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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