What question did this study set out to answer?

To investigate the binding of dexamethasone and its regulatory effect on glucocorticoid receptors in rat placenta.

The Placenta as a Glucocorticoid Target Tissue: Exchange Assay of (3H)-Dexamethasone Binding and Effect of Steroids on Receptor Content

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Randomized trial explores the effect of glucocorticoids on receptor binding in rat placenta, suggesting self-regulation by dexamethasone.

To investigate the binding of dexamethasone and its regulatory effect on glucocorticoid receptors in rat placenta.
Binding of (3H)-dexamethasone was measured using an exchange assay in rat placenta tissues.
Pregnant rats received in vivo treatment with various steroids for four days.
Characteristics of binding, such as maximum binding time and competition with other steroids, were analyzed.
Binding of (3H)-dexamethasone was significantly decreased after dexamethasone treatment compared to other steroids.
High affinity binding sites were identified with a low capacity of 190-250 fmoles/mg protein and a Kd of 10(-8) M.
In basal trophoblast, only dexamethasone displaced the ligand, whereas in labyrinthine zone, multiple steroids competed for binding.

Heller et al. (1983) studied this question.

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