What question did this study set out to answer?

To determine the stability and binding kinetics of glucocorticoid receptors in lung cytosol and assess how sulfhydryl compounds influence receptor preservation.

April 1, 1977Endocrinology

The Role of Sulfhydryl Groups in the Binding of Glucocorticoids by Cytoplasmic Receptors of Lung and Other Mammalian Tissues1

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Authors

JGJonas GranbergSwedish School of Sport and Health Sciences PBPhilip L. BallardUniversity of California, San Francisco

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Implication

Biochemical analysis reveals sulfhydryl reduction stabilizes glucocorticoid receptor binding in mammalian lung cytosol, indicating oxidation causes rapid receptor inactivation in vitro.

Key Points

To determine the stability and binding kinetics of glucocorticoid receptors in lung cytosol and assess how sulfhydryl compounds influence receptor preservation.
Assayed [3H]dexamethasone binding kinetics at 2°C in cytosol preparations of lung and other tissues from adult and fetal rats, mice, hamsters, monkeys, and humans.
Treated cytosol preparations with reducing agents, including dithiothreitol (DTT), mercaptoethanol, thioglycerol, and glutathione, to assess inactivation and reactivation rates.
Measured steroid dissociation rates and evaluated cellular nuclear uptake of hormone complexes at 37°C in dispersed lung cells.
Unbound receptor binding capacity in rat lung cytosol decayed rapidly at 2°C with a t1/2 of 40 minutes, but was stabilized and restored by sulfhydryl agents such as 2 mM DTT (t1/2 = 22 hours).
In the presence of DTT, adult rat lung cytosol specifically bound 0.82 pmol [3H]dexamethasone per mg protein (~19,000 sites per cell) with an equilibrium dissociation constant of 2.5 nM at 2°C.
DTT increased dexamethasone binding in lung cytosol of adult hamsters, adult mice, fetal monkeys, and 11 of 15 adult rat tissues, whereas liver cytosol contained an endogenous heat-stable stabilizing factor.

Cite This Study

Granberg et al. (1977) studied this question.

synapsesocial.com/papers/6a72058235aa2c282ce30ce1 https://doi.org/10.1210/endo-100-4-1160

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Implication

Biochemical analysis reveals sulfhydryl reduction stabilizes glucocorticoid receptor binding in mammalian lung cytosol, indicating oxidation causes rapid receptor inactivation in vitro.

The Role of Sulfhydryl Groups in the Binding of Glucocorticoids by Cytoplasmic Receptors of Lung and Other Mammalian Tissues1

Discussion

Member takes

Implication

Key Points

Cite This Study

The Role of Sulfhydryl Groups in the Binding of Glucocorticoids by Cytoplasmic Receptors of Lung and Other Mammalian Tissues1

Discussion

Member takes

Implication

Key Points

Cite This Study

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