What question did this study set out to answer?

Characterize the active site architecture and catalytic residues of the yeast tRNA:m5C methyltransferase Trm4p and elucidate evolutionary links among 5-methylpyrimidine-generating methyltransferases.

April 28, 2004Nucleic Acids ResearchOpen Access

Sequence-structure-function studies of tRNA:m5C methyltransferase Trm4p and its relationship to DNA:m5C and RNA:m5U methyltransferases

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Authors

JBJanusz M. BujnickiInternational Institute of Molecular and Cell Biology

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Implication

Structural and sequence analysis reveals key catalytic residues and evolutionary transitions in yeast Trm4p, indicating RNA:m5C methyltransferases evolved from RNA:m5U enzymes.

Key Points

Characterize the active site architecture and catalytic residues of the yeast tRNA:m5C methyltransferase Trm4p and elucidate evolutionary links among 5-methylpyrimidine-generating methyltransferases.
Performed sequence alignments and homology modeling of yeast Trm4p (Ncl1p) to map catalytic residues against the crystal structure of homolog PH1374.
Conducted comparative sequence-structure and phylogenetic analyses across RNA:m5C, RNA:m5U, and DNA:m5C methyltransferase families.
Identified two invariant residues essential for Trm4p activity—a lysine in motif I and an aspartate in motif IV—supplementing the established critical cysteines in motifs IV and VI.
Demonstrated that a conserved glutamine residue in motif X is dispensable for methyltransferase catalytic function.
Formulated an evolutionary model indicating RNA:m5C methyltransferases derived from RNA:m5U methyltransferases and subsequently gave rise to DNA:m5C methyltransferases through active-site cysteine substitution and sequence permutation.

Cite This Study

Janusz M. Bujnicki (2004) studied this question.

synapsesocial.com/papers/6a70ff12e36a167817e2d37c https://doi.org/10.1093/nar/gkh564

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Implication

Structural and sequence analysis reveals key catalytic residues and evolutionary transitions in yeast Trm4p, indicating RNA:m5C methyltransferases evolved from RNA:m5U enzymes.

Sequence-structure-function studies of tRNA:m5C methyltransferase Trm4p and its relationship to DNA:m5C and RNA:m5U methyltransferases

Discussion

Member takes

Implication

Key Points

Cite This Study

Sequence-structure-function studies of tRNA:m5C methyltransferase Trm4p and its relationship to DNA:m5C and RNA:m5U methyltransferases

Discussion

Member takes

Implication

Key Points

Cite This Study

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