What question did this study set out to answer?

To examine the cellular mechanisms regulating atriopeptin secretion and processing in response to membrane stretch and calcium modulation in rat myocytes.

March 1, 1989Journal of Clinical InvestigationOpen Access

Stretch-induced atriopeptin secretion in the isolated rat myocyte and its negative modulation by calcium.

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Population

Isolated rat atrial myocytes and fetal or early neonatal ventricular myocytes

Design

Preclinical

Authors

JGJames E. GreenwaldMonsanto (United States)MAMichael ApkonUniversity of Toronto KHKeith A. HruskaWashington University in St. Louis

Discussion

Member takes

Implication

Stretch-stimulated atriopeptin secretion is negatively modulated by intracellular calcium in isolated myocytes; leaves open in vivo relevance to human natriuretic peptide regulation.

Key Points

To examine the cellular mechanisms regulating atriopeptin secretion and processing in response to membrane stretch and calcium modulation in rat myocytes.
Exposed isolated rat atrial myocytes and fetal or early neonatal ventricular myocytes to osmotic stretch and potassium chloride depolarization.
Modulated calcium concentrations using the extracellular chelator EGTA, intracellular chelator BAPTA AM, and calcium ionophore ionomycin.
Characterized the molecular form of secreted atriopeptin following mechanical stimulation.
Osmotic stretch and potassium chloride depolarization stimulated atriopeptin secretion, which was augmented by calcium buffering (EGTA or BAPTA AM) and inhibited by ionomycin-mediated intracellular calcium elevation.
Fetal and neonatal ventricular myocytes actively synthesized and secreted atriopeptin through stretch- and depolarization-responsive pathways similar to atrial myocytes.
Both stretch-stimulated atrial and ventricular myocytes exclusively released the 126-amino acid prohormone.

Structured PICO

Population

Isolated rat atrial myocytes and fetal or early neonatal ventricular myocytes

Intervention

Osmotic stretch, potassium chloride depolarization, and modulation of intra/extracellular calcium (EGTA, BAPTA AM, ionomycin)

Outcome

Atriopeptin secretionsurrogate

Stretch of the myocyte plasma membrane is a major stimulus for atriopeptin secretion, which appears to be negatively modulated by intracellular calcium.

Cite This Study

Greenwald et al. (1989) studied this question.

synapsesocial.com/papers/6a71016575498292b70a7d90 https://doi.org/10.1172/jci113948

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Implication

Stretch-stimulated atriopeptin secretion is negatively modulated by intracellular calcium in isolated myocytes; leaves open in vivo relevance to human natriuretic peptide regulation.

Stretch-induced atriopeptin secretion in the isolated rat myocyte and its negative modulation by calcium.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Stretch-induced atriopeptin secretion in the isolated rat myocyte and its negative modulation by calcium.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider