Key result
Nuclear volume measurement determines cardiomyocyte ploidy with ~99% accuracy compared to FISH.
Effect estimate: F1 score 0.96, accuracy 0.99
Nuclear volume and area provide a highly accurate, fluorescence intensity-independent method for assessing cardiomyocyte ploidy in both isolated cells and tissue sections.
Enables fluorescence-free ploidy assessment in animal cardiomyocytes; leaves open validation and utility in human tissue.
Figure.Evaluation of cardiomyocyte ploidy using nuclear area and volume with high accuracy.A, Representative images of fluorescence in situ hybridization (FISH) using Myc probe and 3-dimensional reconstruction of nuclear volume.Scale bar, 20 m.B and C, Violin plot showing the distribution of nuclear area (B) and volume (C) between diploidy and polyploidy in mouse cardiomyocytes at ages: 14 days (14d), 1 month (1M), 3M, 6M, 12M, and 24M.D and E, F1 score values [D(i), E(i)] and accuracy values [D(ii), E(ii)] correspond to changes in normalized nuclear area and volume, with optimal cutoffs at 1.6 and 1.5, respectively.F, Representative immunofluorescent images of heart tissue sections and 3-dimensional reconstruction of nuclear volume.Scale bar, 50 m.G, The nuclear volume distribution of cardiomyocytes measured in heart tissue sections (n=560) and isolated myocytes (n=583).H, Nuclear volume of (Continued )
No takes yet. Share an insight, caveat, or question.
Yao et al. (2024) studied Cardiomyocyte ploidy. Nuclear volume measurement vs. Fluorescence in situ hybridization (FISH) was evaluated on Accuracy and F1 score of nuclear volume for ploidy assessment (F1 score 0.96, accuracy 0.99). Nuclear volume measurement accurately assessed cardiomyocyte ploidy with an F1 score of 0.96 and 99% accuracy compared to fluorescence in situ hybridization.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: