March 15, 1984Biochemical JournalOpen Access

Isolation of the native form of chicken gizzard myosin light-chain kinase

View Full Paper

Ask AI

Bookmark

Population

Chicken gizzard tissue

Design

Preclinical

Authors

Discussion

Enables proteolysis-free MLCK isolation for functional assays; leaves open applicability to mammalian cardiovascular smooth muscle research.

Population

Chicken gizzard tissue

Intervention

Purification procedure for native form of myosin light-chain kinase

Outcome

Isolation of native myosin light-chain kinase and identification of co-purifying Mr-141000 protein as caldesmon

Describes a novel purification method for native chicken gizzard myosin light-chain kinase that avoids proteolysis and separates it from caldesmon.

Ngai et al. (1984) studied this question.

View Full Paper

Ask AI

Bookmark

Enables proteolysis-free MLCK isolation for functional assays; leaves open applicability to mammalian cardiovascular smooth muscle research.