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October 1, 2000Journal of General Virology

The arterivirus replicase is the only viral protein required for genome replication and subgenomic mRNA transcription

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Population

Equine arteritis virus (EAV) infectious cDNA clone in cell culture

Comparison

Point mutations and deletions in the EAV genome… vs Wild-type EAV

Design

Preclinical

Authors

RMRichard MolenkampErasmus MCHTHans van TolErasmus University RotterdamBRBabette C. D. RozierLeiden University Medical Center

Discussion

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Implication

Replicase-only systems may enable arterivirus reverse genetics; leaves open relevance to natural infection or therapeutic targeting.

Structured PICO

P
Population
Equine arteritis virus (EAV) infectious cDNA clone in cell culture
I
Intervention
Point mutations and deletions in the EAV genome to disrupt structural genes and intraleader ORF
C
Comparator
Wild-type EAV
O
Outcome
Genome replication and subgenomic mRNA transcription

The arterivirus replicase is the only viral protein required for genome replication and subgenomic mRNA transcription, as structural proteins are dispensable.

Cite This Study

Molenkamp et al. (2000) studied this question.

synapsesocial.com/papers/6a711735439bab0cabc372e5https://doi.org/10.1099/0022-1317-81-10-2491
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Open Reading Frame 1a-Encoded Subunits of the Arterivirus Replicase Induce Endoplasmic Reticulum-Derived Double-Membrane Vesicles Which Carry the Viral Replication Complex1999 · 296 citations
  2. 2Identification of a Novel Structural Protein of Arteriviruses1999 · 182 citations
  3. 3An infectious arterivirus cDNA clone: Identification of a replicase point mutation that abolishes discontinuous mRNA transcription1997 · 207 citations
  4. 4Interactions between coronavirus nucleocapsid protein and viral RNAs: implications for viral transcription1988 · 187 citations
  5. 5Defined mutations in the 5' nontranslated sequence of Sindbis virus RNA1990 · 88 citations