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February 4, 1997Proceedings of the National Academy of SciencesOpen Access

An infectious arterivirus cDNA clone: Identification of a replicase point mutation that abolishes discontinuous mRNA transcription

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Population

Baby hamster kidney cells (BHK-21) and the equine arteritis virus (EAV) Bucyrus strain

Design

Preclinical

Authors

LDLeonie C. van DintenLeiden University Medical CenterJBJohan A. den BoonUniversity of Wisconsin–MadisonAWAlfred L.M. WassenaarLeiden University Medical Center

Discussion

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Implication

Separates arterivirus replication from transcription in vitro; leaves open whether replicase mutants enable attenuated vaccines or antivirals in vivo.

Structured PICO

P
Population
Baby hamster kidney cells (BHK-21) and the equine arteritis virus (EAV) Bucyrus strain
I
Intervention
Construction of a full-length cDNA clone (pEAV030) of the EAV genome and electroporation of RNA transcripts into BHK-21 cells
O
Outcome
Infectivity of RNA transcripts and subgenomic mRNA transcription

The construction of an infectious arterivirus cDNA clone demonstrates that arterivirus genome replication and discontinuous mRNA synthesis can be experimentally separated.

Cite This Study

Dinten et al. (1997) studied this question.

synapsesocial.com/papers/6a763ac59aebbbed36614efahttps://doi.org/10.1073/pnas.94.3.991
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Equine arteritis virus subgenomic mRNA synthesis: analysis of leader-body junctions and replicative-form RNAs1996 · 72 citations
  2. 2Proteolytic processing of the replicase ORF1a protein of equine arteritis virus1994 · 159 citations
  3. 3Infectious RNA transcribed from an engineered full-length cDNA template of the genome of a pestivirus1996 · 173 citations
  4. 4The Arterivirus Nsp4 Protease Is the Prototype of a Novel Group of Chymotrypsin-like Enzymes, the 3C-like Serine Proteases1996 · 117 citations
  5. 5Classical swine fever virus: recovery of infectious viruses from cDNA constructs and generation of recombinant cytopathogenic defective interfering particles1996 · 153 citations