A novel technique using a primed-constant infusion of [U-13C]glucose significantly reduces the time to reach equilibrium enrichment of expired CO2.
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May expedite substrate oxidation studies; extends primed-infusion tracer methods to shorter protocols.
We have described a technique whereby the time necessary to reach an equilibrium enrichment of expired CO2 during a primed-constant infusion of [U-13C]glucose was shortened from 7 to 8 h to 1 hour or less. We applied the theory of the primed-constant infusion technique to the bicarbonate pool, with the "constant infusion" of labeled carbon dioxide originating from oxidation of the infused [13C]glucose rather than from a labeled infusion of bicarbonate.
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Allsop et al. (1978) studied this question.
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