What question did this study set out to answer?

To establish and validate a simplified bioassay using unfractionated human serum and JP09 CHO cells expressing recombinant TSH-receptor to measure stimulating and blocking autoantibodies.

March 1, 1998Hormone and Metabolic Research

Application of a Bioassay with CHO Cells for the Routine Detection of Stimulating and Blocking Autoantibodies to the TSH-Receptor

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Authors

NMNG MorgenthalerGILUPI (Germany)IPINA PAMPELLeipzig University GAGabriela AustUniversity Hospital Leipzig

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Implication

Diagnostic validation study demonstrates high sensitivity of a simplified cell bioassay using unfractionated serum, indicating improved clinical detection.

Key Points

To establish and validate a simplified bioassay using unfractionated human serum and JP09 CHO cells expressing recombinant TSH-receptor to measure stimulating and blocking autoantibodies.
Tested unfractionated human serum on JP09 CHO cells transfected with recombinant TSH receptor, measuring cAMP production via radioimmunoassay.
Evaluated 126 patients with Graves' disease, 40 with Hashimoto's thyroiditis, 64 with non-thyroid autoimmune disorders, and 100 healthy controls.
Compared performance against standard PEG precipitation bioassay and commercial TRAK binding assay.
Thyroid stimulating autoantibodies (TSAb) were detected in 107/126 (85%) Graves' disease patients (76/83, 92% in hyperthyroid subgroup), 4/40 (10%) Hashimoto's cases, 1/64 (1.6%) non-thyroid autoimmune controls, and 2/100 (2%) healthy controls.
Combining TSAb and TBII identified 98% of all Graves' disease patients and 99% of the hyperthyroid subgroup, despite a weak correlation between the assays (r = 0.30).
The simplified unfractionated serum assay correlated strongly with the standard PEG precipitation bioassay (r = 0.76, p < 0.001).

Cite This Study

Morgenthaler et al. (1998) studied this question.

synapsesocial.com/papers/6a712afc35aa2c282ce27998 https://doi.org/10.1055/s-2007-978858

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Implication

Diagnostic validation study demonstrates high sensitivity of a simplified cell bioassay using unfractionated serum, indicating improved clinical detection.

Application of a Bioassay with CHO Cells for the Routine Detection of Stimulating and Blocking Autoantibodies to the TSH-Receptor

Discussion

Member takes

Implication

Key Points

Cite This Study

Application of a Bioassay with CHO Cells for the Routine Detection of Stimulating and Blocking Autoantibodies to the TSH-Receptor

Discussion

Member takes

Implication

Key Points

Cite This Study

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