What question did this study set out to answer?

To establish and evaluate a sensitive, practical bioassay for thyroid-stimulating antibodies using FRTL-5 rat thyroid cells and cyclic AMP quantification under hypotonic conditions.

May 1, 1987European Journal of Endocrinology

A sensitive and practical assay for thyroid-stimulating antibodies using FRTL-5 thyroid cells

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Authors

KKK KasagiKyushu University JKJunji KonishiKanazawa University YIYasuhiro IidaGifu University

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Implication

Laboratory study demonstrates high sensitivity of an FRTL-5 cell bioassay for thyroid-stimulating antibodies, indicating reliable diagnostic utility in Graves' disease.

Key Points

To establish and evaluate a sensitive, practical bioassay for thyroid-stimulating antibodies using FRTL-5 rat thyroid cells and cyclic AMP quantification under hypotonic conditions.
Poorly differentiated rat thyroid cells (FRTL-5) were incubated for 2 hours at 37°C with polyethylene glycol-precipitated serum immunoglobulin fractions in NaCl-free Hank's medium.
Released extracellular cAMP was quantified via radioimmunoassay and evaluated across healthy controls and patients with various thyroid disorders (N=146).
Assay sensitivity showed an approximately 30-fold increase in cAMP at 10 mU/l bovine TSH, correlating strongly with porcine (r = 0.918, N = 72) and human (r = 0.830, N = 23) cell assays.
Thyroid-stimulating activity was detected in 100% of patients with hyperthyroid Graves' disease (50/50), recurrent Graves' disease (14/14), and ophthalmic Graves' disease (25/25).
Stimulating activity was present in 37.5% (9/24) of Hashimoto's thyroiditis patients with TSH > 30 mU/l (completely abolished by anti-TSH antibodies), 1/10 simple goitre, 1/10 thyroid cancer, and 0/27 across silent thyroiditis, adenomatous goitre, and adenoma.

Cite This Study

Kasagi et al. (1987) studied this question.

synapsesocial.com/papers/6a712afc35aa2c282ce279a1 https://doi.org/10.1530/acta.0.1150030

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Implication

Laboratory study demonstrates high sensitivity of an FRTL-5 cell bioassay for thyroid-stimulating antibodies, indicating reliable diagnostic utility in Graves' disease.

A sensitive and practical assay for thyroid-stimulating antibodies using FRTL-5 thyroid cells

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Implication

Key Points

Cite This Study

A sensitive and practical assay for thyroid-stimulating antibodies using FRTL-5 thyroid cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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