Thrombin-activated thrombelastography for evaluation of thrombin interaction with thrombin inhibitors

Nonrecalcified whole blood (in vitro model)

Direct thrombin inhibitors (argatroban 3.75 microg/ml, bivalirudin 15 microg/ml, lepirudin 3.0 microg/ml) and indirect inhibitors (heparin cofactor II activator and dermatan disulfate 20 microg/ml) in the presence of 100 nmol/l thrombin

Comparison among different inhibitors and baseline thrombin response without inhibitors

Clot formation parameters (lag time and viscoelasticity) evaluated by thrombelastographysurrogate