Circulation of HAdV-41 with diverse genome types and recombination in acute gastroenteritis among children in Shanghai

Figures

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  Figure 1. Comparative analysis of the hexon and E2A genes. Sequences of genes were aligned and visualized using MEGA6. Position of InDels compared to the reference genome NIVD103 are indicated in the figure.

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  Figure 2. Phylogenetic tree of the five isolates, along with all available HAdV-F isolates and other representative HAdV species. The trees were constructed by neighbor-joining method using MEGA6 with 1,000 bootstrap replicates. Black triangles represent the 5 HAdV-41 isolates in this study. Numbers at tree nodes indicate percentages of bootstrap values. The GenBank accession numbers are contained in the name of isolates.

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  Figure 3. Phylogenetic tree based on the HVRs of the HAdV-41 hexon genes of reference strains from previous studies and our isolates from Shanghai. Forty-three HAdV-41 isolates with partial hexon sequences are also included and indicated with accession numbers and names.

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  Figure 4. The Neighbor-Joining phylogenetic tree showing relationship of five isolates in this study to other adenoviruses inferred from (a) DNA polymerase, (b) hexon gene, (c) long fiber gene, and (d) short fiber gene.

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  Figure 5. Bootscan plots of hexon, long fiber and whole genome sequences of the recombinant isolates. (a) Hexon of SH/2015/D16; (b) genome of SH/2015/D16; (c) hexon of SH/2015/D240; (d) genome of SH/2015/D240; (e) hexon of SH/2015/D363; (f) genome of SH/2015/D363; (g) long fiber of SH/2015/D363; and (h) genome of SH/2015/D187. The analysis of genes is set with a window size of 100 bp and a step size of 20 bp, while whole genomes with a window size of 1000 bp and a step size of 200 bp. The query isolates are listed above each parts of the figure, and the references are colored accordingly in the color schemes under the figure.