Identifies a specific conserved region (region VII) in alpha-like DNA polymerases involved in nucleotide and PPi substrate recognition, where a point mutation confers resistance to acyclovir and foscarnet.
Alerts to possible cross-resistance in herpesvirus therapy; leaves open prevalence and clinical impact in human isolates.
We have shown that a drug-resistant mutant from a clinical isolate of herpes simplex virus contains a single point mutation in the DNA polymerase gene that confers resistance to both acyclovir and foscarnet. The mutated amino acid is located within a distinct conserved region shared among alpha-like DNA polymerases which we designate region VII. We infer that these conserved sequences are directly or indirectly involved in the recognition and binding of nucleotide and PPi substrates.
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Hwang et al. (1992) studied this question.
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