What question did this study set out to answer?

To determine how the expression of cytokines IL-1 and TNF-alpha is regulated across distinct stages of bone marrow-derived macrophage differentiation.

January 1, 1989The Journal of ImmunologyOpen Access

Regulation of IL-1 and TNF- alpha expression during the differentiation of bone marrow derived macrophage.

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Authors

MMMichael J. MyersCenter for Veterinary Medicine JPJ K PullenUnited States Army Medical Research Institute of Infectious Diseases NGN. P. GhildyalCentral Food Technological Research Institute

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Implication

In vitro study demonstrates distinct stage-specific regulation of IL-1 and TNF-alpha in differentiating bone marrow macrophages, indicating independent control of immune affector and effector...

Key Points

To determine how the expression of cytokines IL-1 and TNF-alpha is regulated across distinct stages of bone marrow-derived macrophage differentiation.
Cultured bone marrow-derived macrophages across an in vitro differentiation timeline up to 7 days.
Treated cultures with endotoxin, IFN-gamma, or CSF-1 to assess mRNA and protein expression of IL-1 and TNF-alpha.
Monitored expression profiles of proto-oncogenes c-myc and c-fms alongside transferrin receptor controls.
IL-1 beta mRNA was expressed constitutively by day 3 and peaked 2 to 6 hours after endotoxin exposure, whereas IL-1 alpha mRNA appeared only after endotoxin exposure, with membrane IL-1 appearing at day 7.
TNF-alpha mRNA was constitutively expressed and upregulated by both endotoxin and IFN-gamma, directly coinciding with the acquisition of macrophage tumoricidal activity.
c-myc mRNA showed transient expression on day 3 that briefly rose after endotoxin stimulation, while constitutive c-fms expression declined at day 7 after endotoxin exposure.

Cite This Study

Myers et al. (1989) studied this question.

synapsesocial.com/papers/6a713fe435aa2c282ce28fb7 https://doi.org/10.4049/jimmunol.142.1.153

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Implication

In vitro study demonstrates distinct stage-specific regulation of IL-1 and TNF-alpha in differentiating bone marrow macrophages, indicating independent control of immune affector and effector...

Regulation of IL-1 and TNF- alpha expression during the differentiation of bone marrow derived macrophage.

Discussion

Member takes

Implication

Key Points

Cite This Study

Regulation of IL-1 and TNF- alpha expression during the differentiation of bone marrow derived macrophage.

Discussion

Member takes

Implication

Key Points

Cite This Study

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