June 2, 1987Biochemistry

Nucleotide-induced change in the interaction between the 20- and 26-kilodalton heavy-chain segments of myosin adenosine triphosphatase revealed by chemical cross-linking via the reactive thiol SH2

View Full Paper

Ask AI

Bookmark

Population

Myosin subfragment 1 (S-1)

Design

Preclinical

Authors

THToshiaki HiratsukaAsahikawa Medical College Hospital

Discussion

Member takes

Implication

No clinical implications for myosin-targeted therapies; leaves open the nucleotide-sensitive architecture of the cardiac ATPase site.

Structured PICO

Population

Myosin subfragment 1 (S-1)

Intervention

Chemical cross-linking with bifunctional reagents (p-nitrophenyl iodoacetate and p-nitrophenyl bromoacetate) and exposure to nucleotides

Outcome

Cross-linking between the 20-kDa and 26-kDa segments of the heavy chain via reactive thiol SH2surrogate

This biochemical study suggests that the SH2 thiol of myosin subfragment 1 lies close to both the 26- and 50-kDa segments, supporting the hypothesis that the ATPase site resides where all three segments are contiguous.

Cite This Study

Toshiaki Hiratsuka (1987) studied this question.

synapsesocial.com/papers/6a71421fe5469ee92be1d64f https://doi.org/10.1021/bi00385a034

View Full Paper

Ask AI

Bookmark