March 8, 1988Biochemistry

The myosin (SH2-50-kilodalton fragment) cross-link: location and consequences

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Population

Skeletal myosin subfragment 1 (S-1)

Comparison

-N,N'-p-phenylenedimaleimide cross-linking vs (SH1-SH2)-pPDM-cross-linked S-1 and unmodified S-1

Design

Preclinical

Authors

PCPatrick ChaussepiedCentre National de la Recherche Scientifique MMManuel F. MoralesUniversidad de Las Palmas de Gran Canaria RKRidha KassabCentre National de la Recherche Scientifique

Discussion

Member takes

Implication

Identifies myosin cross-link site boosting actin affinity; hypothesis-generating for contractile regulation, leaves clinical translation open.

Structured PICO

Population

Skeletal myosin subfragment 1 (S-1)

Intervention

(Cys697-Cys540)-N,N'-p-phenylenedimaleimide (pPDM) cross-linking

Comparator

(SH1-SH2)-pPDM-cross-linked S-1 and unmodified S-1

Outcome

Identification of the SH chi thiol location and characterization of spectral, enzymatic, and actin-binding properties of the cross-linked S-1surrogate

The study identifies the specific location of a novel interthiol cross-link in skeletal myosin and demonstrates its significant effects on actin binding affinity.

Cite This Study

Chaussepied et al. (1988) studied this question.

synapsesocial.com/papers/6a71421fe5469ee92be1d64d https://doi.org/10.1021/bi00405a059

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Implication

Identifies myosin cross-link site boosting actin affinity; hypothesis-generating for contractile regulation, leaves clinical translation open.

The myosin (SH2-50-kilodalton fragment) cross-link: location and consequences

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The myosin (SH2-50-kilodalton fragment) cross-link: location and consequences

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider