What question did this study set out to answer?

To examine the stability, cellular distribution, and different functional states of clearing-factor lipase in intact adipose tissue and isolated fat cells from fed and starved rats.

April 1, 1969Biochemical JournalOpen Access

Clearing-factor lipase in adipose tissue. Distinction of different states of the enzyme and the possible role of the fat cell in the maintenance of tissue activity

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Population

Epididymal adipose tissue and isolated fat cells from fed and starved rats

Comparison

Incubation at 37 degrees in albumin solution… vs Intact tissue vs isolated cells; fed vs starved…

Design

Preclinical

Authors

VCVincent J. CunninghamForestry Commission England DRD. S. RobinsonDartmouth College

Discussion

Member takes

Implication

Adipocyte isolation may underestimate lipase activity in fed states; leaves open nutritional regulation of extracellular enzyme pools.

Key Points

To examine the stability, cellular distribution, and different functional states of clearing-factor lipase in intact adipose tissue and isolated fat cells from fed and starved rats.
Incubated intact epididymal adipose tissue and isolated fat cells from fed and starved rats at 37°C in albumin or serum-based media.
Separated fat cells and stromal-vascular cells using collagenase digestion and assessed the response of lipase activity to actinomycin.
Less than 20% of intact adipose tissue clearing-factor lipase activity from fed rats was retained after collagenase isolation, whereas tissue activity from starved rats resisted collagenase inactivation.
Incubation of isolated fat cells in serum-based medium produced a progressive increase in clearing-factor lipase activity that was enhanced by actinomycin, with no rise observed in stromal-vascular cells.
Enzyme activity in intact fed tissue dropped rapidly at 37°C to a low, stable baseline, while activity in starved intact tissue and isolated fat cells remained stable.

Structured PICO

Population

Epididymal adipose tissue and isolated fat cells from fed and starved rats

Intervention

Incubation at 37 degrees in albumin solution, collagenase treatment, and incubation in serum-based medium with or without actinomycin

Comparator

Intact tissue vs isolated cells; fed vs starved rats

Outcome

Clearing-factor lipase activitysurrogate

The study demonstrates that clearing-factor lipase activity in adipose tissue exists in different states depending on feeding status, and much of the activity in fed rats is lost during collagenase isolation of fat cells.

Cite This Study

Cunningham et al. (1969) studied this question.

synapsesocial.com/papers/6a71423b35aa2c282ce29207 https://doi.org/10.1042/bj1120203

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