January 1, 1987The Journal of Biochemistry

Selective Fluorescent Labeling of the 50-, 26-, and 20-Kilodalton Heavy Chain Segments of Myosin ATPase1

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Population

Myosin subfragment-1 (S-1) ATPase

Design

Preclinical

Authors

THToshiaki HiratsukaAsahikawa Medical College Hospital

Discussion

Member takes

Implication

May advance myosin subdomain research tools; leaves open validation in human cardiovascular models.

Structured PICO

Population

Myosin subfragment-1 (S-1) ATPase

Intervention

Fluorescent labeling with 7-Diethylamino-3-(4'-isothiocyanatophenyl)-4-methylcoumarin (CPI), rhodamine B isothiocyanate (RITC), and 4-bromomethyl-6,7-dimethoxycoumarin (BDMC)

Outcome

Selective labeling of the 50-, 26-, and 20-kDa segments of the S-1 heavy chain and effects on ATPase activitiessurrogate

CPI, RITC, and BDMC can be used as selective fluorescent labels for the three segments of the myosin S-1 heavy chain.

Cite This Study

Toshiaki Hiratsuka (1987) studied this question.

synapsesocial.com/papers/6a71429735aa2c282ce29243 https://doi.org/10.1093/oxfordjournals.jbchem.a122015

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Selective Fluorescent Labeling of the 50-, 26-, and 20-Kilodalton Heavy Chain Segments of Myosin ATPase1

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Selective Fluorescent Labeling of the 50-, 26-, and 20-Kilodalton Heavy Chain Segments of Myosin ATPase1

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider