February 1, 1985Biochemistry

Effect of tryptic cleavage on the stability of myosin subfragment 1. Isolation and properties of the severed heavy-chain subunit

View Full Paper

Ask AI

Bookmark

Population

Myosin subfragment 1 (SF1)

Comparison

Tryptic cleavage vs Undigested SF1

Design

Preclinical

Authors

MBMorris BurkeCase Western Reserve University VKVedhachalam KamalakannanUniversity of Madras

Discussion

Member takes

Implication

Delineates myosin heavy chain domains for ATPase function; extends SF1 biochemistry but leaves open relevance to intact cardiac muscle.

Structured PICO

Population

Myosin subfragment 1 (SF1)

Intervention

Tryptic cleavage

Comparator

Undigested SF1

Outcome

Stability of subunit interactions and ATPase propertiessurrogate

Tryptic cleavage destabilizes myosin SF1 subunit interactions, but the severed heavy chain retains ATPase activity and actin affinity independent of the alkali light chain.

Cite This Study

Burke et al. (1985) studied this question.

synapsesocial.com/papers/6a71437f6ceb2bbd16e01c12 https://doi.org/10.1021/bi00325a006

View Full Paper

Ask AI

Bookmark

Effect of tryptic cleavage on the stability of myosin subfragment 1. Isolation and properties of the severed heavy-chain subunit

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Effect of tryptic cleavage on the stability of myosin subfragment 1. Isolation and properties of the severed heavy-chain subunit

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider