What question did this study set out to answer?

To determine the roles of human cytomegalovirus major immediate-early gene products, specifically IE1 and IE2, in transcriptional activation and autoregulation.

April 1, 1988Journal of VirologyOpen Access

trans-activation and autoregulation of gene expression by the immediate-early region 2 gene products of human cytomegalovirus

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Authors

MPMarie C. PizzornoBucknell University POPeter O’HareImperial College London LSLinlin ShaWenzhou Medical University

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Implication

In vitro assays reveal that cytomegalovirus IE2 proteins trans-activate heterologous genes while repressing their own promoter, indicating a dual regulatory role in viral infection.

Key Points

To determine the roles of human cytomegalovirus major immediate-early gene products, specifically IE1 and IE2, in transcriptional activation and autoregulation.
Conducted transient chloramphenicol acetyltransferase reporter assays in Vero cells cotransfected with the HCMV Towne HindIII C fragment and target viral or host promoters.
Performed inactivation mapping, subcloning, internal deletions, and terminator-codon insertion mutagenesis across the 7.1-kilobase IE1 and IE2 gene regions (exons 1 to 5).
Cotransfection stimulated heterologous viral and cellular promoters 30- to 800-fold, while specifically repressing the homologous HCMV IE68 promoter.
Terminator mutations in IE2 exon 5 abolished both trans-activation and autoregulation, whereas mutations in IE1 exon 4 had no effect on either function.
Isolated expression of IE2 exon 5 specifically down-regulated IE68 promoter activity but failed to trans-activate heterologous target promoters.

Cite This Study

Pizzorno et al. (1988) studied this question.

synapsesocial.com/papers/6a714e3d87f921057128288a https://doi.org/10.1128/jvi.62.4.1167-1179.1988

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Implication

In vitro assays reveal that cytomegalovirus IE2 proteins trans-activate heterologous genes while repressing their own promoter, indicating a dual regulatory role in viral infection.

trans-activation and autoregulation of gene expression by the immediate-early region 2 gene products of human cytomegalovirus

Discussion

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Implication

Key Points

Cite This Study

trans-activation and autoregulation of gene expression by the immediate-early region 2 gene products of human cytomegalovirus

Discussion

Member takes

Implication

Key Points

Cite This Study

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