January 1, 1987Journal of VirologyOpen Access

Comparison of upstream sequence requirements for positive and negative regulation of a herpes simplex virus immediate-early gene by three virus-encoded trans-acting factors

View Full Paper

Ask AI

Bookmark

Population

Short-term cotransfection system with recombinant chloramphenicol acetyltransferase target genes and intact…

Comparison

Deletion mutants of the IE175K… vs Parental construct containing sequences to -1900

Design

Preclinical

Authors

Discussion

Delineates separate HSV IE promoter elements; extends viral gene regulation mapping but leaves open in vivo relevance.

Population

Short-term cotransfection system with recombinant chloramphenicol acetyltransferase (CAT) target genes and intact genes for regulatory proteins

Intervention

Deletion mutants of the IE175K promoter-regulatory region and trans-acting factors (Vmw65, IE110K, IE175K)

Comparator

Parental construct containing sequences to -1900

Outcome

Gene expression levels (activation or repression)surrogate

The study maps the distinct upstream sequence requirements for positive and negative regulation of the HSV IE175K gene by viral trans-acting factors.

O’Hare et al. (1987) studied this question.

View Full Paper

Ask AI

Bookmark