What question did this study set out to answer?

To determine the extracellular function and localization of platelet-derived factor XIII-A during thrombus formation and stabilization against fibrinolysis.

October 21, 2014BloodOpen Access

Functional factor XIII-A is exposed on the stimulated platelet surface

View Full Paper

Ask AI

Bookmark

To determine the extracellular function and localization of platelet-derived factor XIII-A during thrombus formation and stabilization against fibrinolysis.
Formed Chandler model thrombi using factor XIII (FXIII)-depleted plasma reconstituted with platelets, platelet fractions, or neutralizing antibodies.
Assessed the requirement of platelet activation, transglutaminase activity, and α2-antiplasmin (α2AP) on thrombus resistance to lysis.
Visualized and quantified FXIII-A surface exposure on platelets stimulated with thrombin and collagen using fluorescence confocal microscopy and flow cytometry.
Platelets stabilized FXIII-depleted thrombi against fibrinolysis via a transglutaminase-dependent and α2AP-dependent mechanism, though the surface activity decayed within 1 hour.
Inhibition of platelet activation eliminated thrombus stabilization, while isolated membrane and cytoplasmic fractions from unstimulated platelets both functionally protected thrombi from lysis.
Stimulation with thrombin and collagen induced maximal exposure of FXIII-A, localized to distinct protruding caps on phosphatidylserine-positive platelet membranes.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

In vitro experimental study demonstrates that activated platelets expose functional factor XIII-A to stabilize thrombi against fibrinolysis, highlighting an externalized antifibrinolytic mechanism.

Cite This Study

Mitchell et al. (2014) studied this question.

synapsesocial.com/papers/6a715794ac440176ef2a0694 https://doi.org/10.1182/blood-2014-06-583070

View Full Paper

Ask AI

Bookmark

Implication

In vitro experimental study demonstrates that activated platelets expose functional factor XIII-A to stabilize thrombi against fibrinolysis, highlighting an externalized antifibrinolytic mechanism.

Functional factor XIII-A is exposed on the stimulated platelet surface

Key Points

Discussion

Implication

Cite This Study

Functional factor XIII-A is exposed on the stimulated platelet surface

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider