What question did this study set out to answer?

To identify the cellular origin of plasma factor XIII-A and determine the subcellular mechanisms mediating its nonclassical secretion from monocyte-macrophages.

January 20, 2010Blood

Association of coagulation factor XIII-A with Golgi proteins within monocyte-macrophages: implications for subcellular trafficking and secretion

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To identify the cellular origin of plasma factor XIII-A and determine the subcellular mechanisms mediating its nonclassical secretion from monocyte-macrophages.
Measured plasma FXIII-A levels in thrombocytopenic mouse models (Bcl-x(Plt20/Plt20) and Mpl(-/-)).
Performed [35S] metabolic pulse-chase labeling in THP-1 cells under basal conditions and cellular stress induced by interleukin-1 beta release.
Used immunofluorescence microscopy in THP-1 cells and human monocyte-derived macrophages to assess FXIII-A colocalization with Golgi and endoplasmic reticulum markers.
Thrombocytopenic mice showed unchanged plasma FXIII-A levels, indicating that nucleated cells, rather than platelets, provide the primary source of circulating FXIII-A.
Metabolic labeling showed no detectable secretion of [35S]-FXIII-A from THP-1 cells under baseline conditions or during stress-induced interleukin-1 beta release.
FXIII-A localized adjacent to the plasma membrane and within podosomes, colocalizing with GM130-positive vesicles and TGN46 rather than protein disulphide isomerase.

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Implication

Laboratory study reveals factor XIII-A associates with Golgi matrix proteins in monocyte-macrophages, indicating a nonclassical vesicle transport pathway for cellular secretion.

Cite This Study

Cordell et al. (2010) studied this question.

synapsesocial.com/papers/6a74719d0081fe7b05dbd3d9 https://doi.org/10.1182/blood-2009-08-231316

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Implication

Laboratory study reveals factor XIII-A associates with Golgi matrix proteins in monocyte-macrophages, indicating a nonclassical vesicle transport pathway for cellular secretion.

Association of coagulation factor XIII-A with Golgi proteins within monocyte-macrophages: implications for subcellular trafficking and secretion

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Discussion

Implication

Cite This Study

Association of coagulation factor XIII-A with Golgi proteins within monocyte-macrophages: implications for subcellular trafficking and secretion

Key Points

Discussion

Implication

Cite This Study

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